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Literature summary extracted from

  • Gaweska, H.M.; Taylor, A.B.; Hart, P.J.; Fitzpatrick, P.F.
    Structure of the flavoprotein tryptophan 2-monooxygenase, a key enzyme in the formation of galls in plants (2013), Biochemistry, 52, 2620-2626.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.3 expressed in Escherichia coli as a His-tagged fusion protein Pseudomonas savastanoi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.12.3 determined by X-ray diffraction methods to a resolution of 1.95 A. The overall structure of the protein shows that it has the same fold as members of the monoamine oxidase family of flavoproteins, with the greatest similarities to the L-amino acid oxidases Pseudomonas savastanoi

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.3 K365M mutation decreases the kcat and kcat/Km (L-tryptophan) values by 60000- and 2 millionfold compared to wild-type, respectively Pseudomonas savastanoi
1.13.12.3 W466F mutation decreases the kcat value less than 2fold and the kcat/Km (L-tryptophan) value only 5fold compared to wild-type Pseudomonas savastanoi
1.13.12.3 W466M mutation results in an enzyme lacking flavin and detectable activity Pseudomonas savastanoi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.3 0.04
-
 L-tryptophan pH 8.3, 25°C, wild-type Pseudomonas savastanoi
1.13.12.3 0.13
-
 L-tryptophan pH 8.3, 25°C, mutant W466F Pseudomonas savastanoi
1.13.12.3 1.2
-
 L-tryptophan pH 8.3, 25°C, mutant K365M Pseudomonas savastanoi
1.13.12.3 90
-
 O2 pH 8.3, 25°C, wild-type Pseudomonas savastanoi
1.13.12.3 140
-
 O2 pH 8.3, 25°C, mutant W466F Pseudomonas savastanoi

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.3 Pseudomonas savastanoi P06617
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.3 L-tryptophan + O2
-
 Pseudomonas savastanoi indole-3-acetamide + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.3 tryptophan 2-monooxygenase
-
 Pseudomonas savastanoi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.3 25
-
 assay at Pseudomonas savastanoi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.12.3 0.00022
-
 L-tryptophan pH 8.3, 25°C, cosubstrate: O2, mutant K365M Pseudomonas savastanoi
1.13.12.3 9.2
-
 L-tryptophan pH 8.3, 25°C, cosubstrate: O2, mutant W466F Pseudomonas savastanoi
1.13.12.3 13.2
-
 L-tryptophan pH 8.3, 25°C, cosubstrate: O2, wild-type Pseudomonas savastanoi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.3 8.3
-
 assay at Pseudomonas savastanoi

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.3 flavin
-
 Pseudomonas savastanoi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.12.3 0.00019
-
 L-tryptophan pH 8.3, 25°C, mutant K365M Pseudomonas savastanoi
1.13.12.3 70
-
 O2 pH 8.3, 25°C, mutant W466F Pseudomonas savastanoi
1.13.12.3 72
-
 L-tryptophan pH 8.3, 25°C, mutant W466F Pseudomonas savastanoi
1.13.12.3 140
-
 O2 pH 8.3, 25°C, wild-type Pseudomonas savastanoi
1.13.12.3 360
-
 L-tryptophan pH 8.3, 25°C, wild-type Pseudomonas savastanoi